Prospect of developing a novel potent Antioxidant from an Antidepressant Agent

Shauroseni Palchoudhuri1,Sanchayita Debnath2 , Musfiqua Mookerjee3 and Sujata G.Dastidar1

1Department of Microbiology, Herbicure Healthcare Bio-Herbal Research Foundation. Saraldighi (E), Boral, Kolkata 700154, India

2Department of Botany, Sree Chaitannya College, Habra, 24 Parganas (N), West Bengal, India

3Department of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata, India.

ABSTRACT

Objective: Worldwide scientific investigations strongly suggest the urgent need of antioxidants in preventing the oxidative stress disorders triggered by reactive oxygen species (ROS) and free radicals. Based on multiplicity of action in pharmacological compounds, this study has been directed to search for antioxidant capacity in the antidepressant drug doxepin, having structural similarity with the known antioxidant flavonoid, quercetin.

Methods:Standard spectrophotometric procedures like Total antioxidant capacity assay (TAC assay), Ferric ion and cupric ion reducing power assays (FRAP& CUPRAC assay), Ferrous ion chelating activity assay, hydrogen peroxide and nitric oxide radical scavenging assays were conducted and the results were statistically analysed.

Results: The outcomes showed the antioxidant capacity of the drug to increase stably in a dose-dependent manner for TAC, FRAP and CUPRAC assays. Also almost similar results were observed in case of the ferrous ion chelating activity assay. Though, doxepin revealed a strong nitric oxide scavenging activity in all its concentrations, however, it showed a positive hydrogen peroxide scavenging activity only at highest concentration.

Conclusion: Thus, in this way this study enhances the scope of developing novel antioxidants from already known pharmaceutical compounds that are in routine therapeutic usage.

Keywords: Free radicals; Antioxidants; Doxepin, TAC assay; Nitric oxide scavenging activity

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