Phytochemical characterisation and evaluation of the in-vitro antioxidant profile and antibacterial effect of extracts of Amorphophallus paeoniifolius(Dennst.)

Nikhil Kumar1, Raja Chakraverty2, Debraj Mondal1, Pranabesh Chakraborty1*

1Department of Pharmaceutical Technology. Bengal School of Technology (A College of Pharmacy) Sugandha, Delhi Road, Hooghly-712102.

 

2ICMR Research Fellow, IPGME&R-SSKM Hospital.244 A.J.C Bose Road. Kolkata-700 020.

 

Corresponding author: pchakroborty@rediffmail.com

 

ABSTRACT

According to the World Health Organisation (WHO),herbal drugs are being used by nearly 80% of the indigenouspopulation all over the world. In this context, the present study attempts to evaluate theantioxidant and innate antibacterial effect of ethanolic and hydro-alcoholic extracts of tubers of Amorphophallus paeoniifoliusbelonging to the family Araceae. For the purpose of the study the identification of the plant was followed by infrared spectroscopic studies of the crude extracts followed by phytochemical screening. Subsequently, in-vitro antioxidant activity was evaluated based on the standardised DPPH (1.1-Diphenylpicrylhydrazyl) and Nitric oxide (NO) scavenging tests.In-vitro antibacterial effect of the plant was screened using the cup-plate method using ATCC strains of Staphylococcus aureus and Bacillus subtilis. The zones of inhibition was subsequently compared to that ofstandard antibiotics. The findings of the antioxidant assay depicts that the tubers of this herb has antioxidant activity compared to the IC50 value of ascorbic acid. These findings of the in-vitro assays and the antibacterial study support the hypothesis that hydro-alcoholic extracts of tubers Amorphophallus paeoniifoliushas antioxidant and antibacterial effects. However, larger studies are required to corroborate these preliminary findings.

 

Keywords:Amorphophallus paeoniifolius, phytochemical screening, DPPH assay, antibacterial

Loader Loading...
EAD Logo Taking too long?

Reload Reload document
| Open Open in new tab

Download

Leave a Reply

Your email address will not be published. Required fields are marked *